Work

RNA polymerase II transcription initiation in holo-TFIID-depleted mouse embryonic stem cells

Year: 2024

Type: article

Abstract: The recognition of core promoter sequences by TFIID is the first step in RNA polymerase II (Pol II) transcription initiation. Metazoan holo-TFIID is a trilobular complex, composed of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs). Why and how TAFs are necessary for the formation of TFIID domains and how they contribute to transcription initiation remain unclear. Inducible TAF7 or TAF10 depletion, followed by comprehensive analysis of TFIID subcomplex formation, chromatin binding, and nascent transcription in mouse embryonic stem cells, result in the formation of a TAF7-lacking TFIID or a minimal core-TFIID complex, respectively. These partial complexes support TBP recruitment at promoters and nascent Pol II transcription at most genes early after depletion, but importantly, TAF10 is necessary for efficient Pol II pausing. We show that partially assembled TFIID complexes can sustain Pol II transcription initiation but cannot replace holo-TFIID over several cell divisions and/or development.

Source: Cell Reports

DOI: 10.1016/j.celrep.2024.114791

Authors: Vincent Hisler, Paul Bardot, Dylane Detilleux, Andrea Bernardini, Matthieu Stierle, Emmanuel Garcia Sanchez, Claire Richard, Lynda Hadj Arab, Cynthia Ehrhard, Bastien Morlet, Yavor Hadzhiev, Matthieu Jung, Stéphanie Le Gras, Luc Négroni, Ferenc Müller, László Tora, Stéphane D. Vincent

Institutions: Centre National de la Recherche Scientifique, Inserm, Institut de génétique et de biologie moléculaire et cellulaire, Université de Strasbourg, University of Birmingham

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,460

Funders: National Science Foundation, Agence Nationale de la Recherche, Fondation pour la Recherche Médicale, Université de Strasbourg, National Institutes of Health, National Institute of General Medical Sciences

Awards: Structural and functional study of the human ATAC co-activator complex, Biological significance of the specific transcription machinery during oocyte growth in the mouse, Par-delà les frontières, l'Université de Strasbourg, Organisation et montée en puissance d'une Infrastructure Nationale de Génomique, G2659019386, Façonner les talents en formation et en recherche à l'Université de Strasbourg, EFRI CEE: DNA origami tools to engineer chromatin structure and function in live cells, G2980570810, G3010527862, Epigenetic Complex Assembly in Space and Time, Integrative Molecular and Cellular Biology, G4779185514, G5501925015, Functioning and dysfunctioning of the transcription/repair factor TFIIH, Structure and function of endogenous TFIID-containing complexes, G6405104111, G6803667113, G7240651115, G8144793849, Mechanisms of chromatin regulation of transcription

OpenAlex ID: W4402994959

FWCI: 0.693

Cites: 90

Cited by: 6

Related to: 10