Work

Expression and drought functional analysis of one circRNA PecircCDPK from moso bamboo (Phyllostachys edulis)

Year: 2024

Type: article

Abstract: Drought stress can affect the growth of bamboo. Circle RNAs (CircRNAs) have been found to play a role in drought stress in plants, but their function in moso bamboo is not well understood. In previous studies, we observed that under drought stress, the expression of some circRNAs were altered and predicted to be involved in calcium-dependent protein kinase phosphorylation, as indicated by KEGG enrichment analysis. In this study, we cloned a circRNA called PecircCDPK in moso bamboo that is responsive to drought stress. To further investigate its function, we constructed an overexpression vector using flanking intron sequences supplemented by reverse complementary sequences. When this vector was transferred to Arabidopsis plants, we observed that the roots of the transgenic lines were more developed, the water loss rate decreased, the stomata became smaller, and the activity of antioxidant enzymes increased under drought stress. These findings suggest that overexpression of PecircCDPK can enhance the drought resistance of Arabidopsis thaliana, providing valuable insights for the breeding of moso bamboo with improved resistance to drought.

Source: PeerJ

DOI: 10.7717/peerj.18024

Authors: Yiqian Li, Wen Xia, Ying Li, Xueping Li

Institutions: International Bamboo and Rattan Organization, International Center for Bamboo and Rattan

Language: English

Topic: Circular RNAs in diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $1,395