Work

A chitosan-based near-infrared ratiometric fluorescent nanoprobe created by molecular assembly with applications in hypochlorous acid detection in live mouse

Year: 2024

Type: article

Abstract: Hypochlorous acid (HClO/ClO − ) is a key reactive oxidative species (ROS) in the body. The HClO/ClO − concentrations are imbalanced during cancer formation due to the ROS stress response. This paper introduces a novel chitosan-based self-calibration fluorescent nanoprobe (ChCyNil) constructed by molecular assembly for the ratiometric detection of HClO/ClO − . Two chromophores with different fluorescence characteristics and HClO/ClO − sensitivity were labeled on chitosan, and nanoparticles were prepared by a self-assembly strategy for HClO/ClO − detection. ChCyNil exhibits several advantages, such as dual near-infrared emissions at 670 nm and 845 nm, tunable fluorescence intensity , self-calibration fluorescence, and good biocompatibility , improving its accuracy in HClO/ClO − detection. Our study confirmed that ChCyNil exhibits a well-assembled spheroidal nanostructure and good photophysical properties in solution. The fluorescence imaging properties were further proved by detecting endogenous HClO/ClO − produced by LPS/PMA stimuli in cells and zebrafish. In addition, ChCyNil was used to detect the fluorescence behavior of HClO/ClO − in tumors of live mice. The successful design and fabrication of ChCyNil have presented a new strategy for constructing detection tools with improved fluorescence properties for HClO/ClO − in live animals.

Source: International Journal of Biological Macromolecules

DOI: 10.1016/j.ijbiomac.2024.136165