Year: 2024
Type: article
Abstract: The significance of easily detecting rare earth elements (REEs) has increased due to the growing demand for REEs. Addressing this need, we present an innovative electrochemical biosensor, focusing on cerium as a model REE. This biosensor utilizes a modified EF‐hand loop peptide sequence, incorporating cysteine for covalent attachment to a gold working electrode and tyrosine as an electrochemically active amino acid. The sensor was designed such that binding to cerium induces a conformational change in the peptide, affecting tyrosine's proximity to the electrode surface, modulating the current. A calibration curve was generated from cyclic voltammetry current peaks at ~0.55–0.65 V versus a silver pseudo‐reference electrode, with cerium concentrations ranging from 0 to 67 μM in artificial urine. The sensor exhibited a biologically relevant limit of detection of 35 μM and a sensitivity of −0.0024 ± 0.002 (μA μM)−1. These findings offer insights into designing peptide sequences for electrochemical biosensing.
Source: AIChE Journal
DOI: 10.1002/aic.18620
Institution: Case Western Reserve University
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,940
OpenAlex ID: W4403048271