Work

NMR assignment of the conserved bacterial DNA replication protein DnaA domain IV

Year: 2024

Type: article

Abstract: Chromosomal replication is a ubiquitous and essential cellular process. In bacteria, the master replication initiator DnaA plays a key role in promoting an open complex at the origin (oriC) and recruiting helicase in a tightly regulated process. The C-terminal domain IV specifically recognises consensus sequences of double-stranded DNA in oriC, termed DnaA-boxes, thereby facilitating the initial engagement of DnaA to oriC. Here, we report the 13Cβ and backbone 1H, 15N, and 13C chemical shift assignments of soluble DnaA domain IV from Bacillus subtilis at pH 7.6 and 298 K.

Source: Biomolecular NMR Assignments

DOI: 10.1007/s12104-024-10206-1

Authors: Alexander Nguyen Abrams, Geoff Kelly, Julia Hubbard

Institutions: Newcastle University, The Francis Crick Institute, Medical Research Council

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,690

Funders: Wellcome Trust, Francis Crick Institute, Cancer Research UK, Medical Research Council