Work

New insight into Clostridium butyricum-ferroferric oxide hybrid system in exogenous carbon dioxide-assisted anaerobic fermentation for acetate and butyrate production

Year: 2024

Type: article

Abstract: Mixotrophic cultivation, utilizing both gas and organic substances, is commonly employed to minimize the carbon loss during anaerobic fermentation of bulk chemicals. Herein, a novel Clostridium butyricum -ferroferric oxide (Fe 3 O 4 ) hybrid system, enhanced by exogenous carbon dioxide (CO 2 ), was proposed to improve carbon recovery and optimize metabolite production. The results demonstrated that exogenous CO 2 improved metabolite selectivity towards acetate/butyrate, while also accelerating CO 2 fixation. Compared to pure Clostridium butyricum , the hybrid system significantly increased carbon conversion to primary metabolites, boosting butyrate and acetate production by 18.7 % and 18.4 %, respectively. Enzyme activity assays revealed that Fe 3 O 4 and exogenous CO 2 acted synergistically, enhancing the activities of key enzymes involved in CO 2 assimilation. Additionally, Fe 3 O 4 facilitated intra- and extracellular electron transfer, further improving the fermentation process. This study offers new insights into the combined effects of exogenous CO 2 and Fe 3 O 4 on anaerobic fermentation, providing an efficient strategy for carbon recovery and selective acetate/butyrate production.

Source: Bioresource Technology

DOI: 10.1016/j.biortech.2024.131576