Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies
Year: 2024
Type: article
Abstract: Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity to IFNs than a commonly used alternative biological reporter system and allows for a rapid and simple live-cell workflow for detecting low titer amounts of neutralizing anti-IFN antibodies.
Source: European Journal of Immunology
Authors: Kevin Groen, Roger Kuratli, Lauren Sar, Andri Vasou, Michael Huber, David J. Hughes, Benjamin G. Hale
Institutions: University of Zurich, University of St Andrews
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,330
Awards: G769000348, G8452176403
OpenAlex ID: W4403158586