Work

FTX promotes esophageal cancer progression and desensitizes esophageal cancer cells to ionizing radiation by microRNA-99a/b-3p/WEE1/ERCC1 axis

Year: 2024

Type: article

Abstract: Objectives This study was conducted to uncover the underlying cellular and molecular mechanisms of five prime to Xist (FTX) dysregulation in esophageal cancer (EC). Methods The gene expression in tumor tissues was detected using western blot, immunohistochemistry, and quantitative real-time polymerase chain reaction (qPCR). The dual-luciferase reporter and RNA FISH assays confirmed the interaction between miRNA and target genes. Mouse models for Xenograft and lung metastasis were used to assess EC cell tumorigenesis and metastasis. Results This study finds that up-regulated FTX in patients with esophageal cancer correlates with poor clinical outcomes. Silencing FTX inhibits esophageal cancer cell growth and migration in vitro and tumor metastasis in vivo. miR-99a/b-3p sensitizes esophageal cancer cells to ionizing radiation by WEE1 (Wee1-like protein kinase) and ERCC1 (excision repair cross-complementation group 1) in vitro. Conclusions FTX promotes the malignant biological phenotype of esophageal cancer cells. Mechanistically, FTX acts as a ceRNA to regulate the transcription of WEE1 and ERCC1 by sponging hsa-miR-99a/b-3p.

Source: ONCOLOGIE

DOI: 10.1515/oncologie-2024-0250

Authors: Yangjie Xu, Chaoming Huang, Zijuan Mao, Yuanyuan Fu, Qiongyu Hao, Zhenghua Fei

Institutions: Wenzhou Medical University, First Affiliated Hospital of Wenzhou Medical University, University of Hawaiʻi at Mānoa, Charles R. Drew University of Medicine and Science

Language: English

Topic: Cancer-related molecular mechanisms research

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,082