Auxiliaries in Native Chemical Ligation: Exploring the Reactivity Landscape with new Scaffolds
Year: 2024
Type: conference-abstract
Abstract: The auxiliary-mediated native chemical ligation (NCL) serves as an essential tool in expanding the scope of NCL beyond cysteine, allowing for the selection of any amide bond for retrosynthetic scission of proteins.While long conceived as limited to "easy junctions" like Gly-Gly, our newest generation of auxiliaries are capable of tackling reactions of two β-branched amino acids and are feasible for ligation with phosphorylated or glycosylated amino acids at pH 7 or in acidic medium. Auxiliaries in Native Chemical Ligation: Exploring the Reactivity Landscape with new Scaffolds❑ auxiliary-mediated NCL can be enhanced via intramolecular base catalysis [1, 2, 3] A new cleavage paradigm Acid-base catalysis benzyl-type MPE MPyEGly-Gly
Source: European Peptide Symposium (EPS)
Institution: Humboldt-Universität zu Berlin
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W4403190864