Development And Validation Of RP-HPLC Method For Estimation Of Some Antiviral Drugs Using Aqbd Approach
Year: 2024
Type: article
Abstract: A precise and robust method was developed method for the estimation of some antiviral drugs i.e. Acyclovir (ACY), Abacavir (ABA), Sofosbuvir (SOF) and Lopinavir (LPV) in bulk and pharmaceutical dosage form. The Method used Agilent 1260 Infinity II model HPLC with DAD detector and Phenomenex Kinetex XB-C8 (150 mm × 4.6 mm, 5mm). The Mobile phase combination used was 0.2% Perchloric acid and Acetonitrile [50:50]. Flow rate at 0.5ml/min and wavelength at 210 nm with run time of 10 minutes. The retention time of Acyclovir, Abacavir, Sofosbuvir and Lopinavir peaks was at 2.9minutes, 3.29 minutes, 4.49 minutes and 14.19minutes, respectively. The developed method was validated according to ICH Q2 (R1) guidelines. % RSD for Instrument precision, Method Precision and Intermediate precision was found to be below 2.0% for the antiviral drugs. The Intra& Inter-day precision for Acyclovir, Abacavir, Sofosbuvir and Lopinavir had a %RSD of less than 2.0% for all the APIs. Method was linear and accurate for concentration range of 40-60 mg/ml for all four APIs. For accuracy, maximum % RSD of ACY at 80% which was 0.21%, for ABA at 100% which was 0.12%, for SOF and LPV at 120% which was 0.39% and 0.34% respectively. The method was found to be robust for simultaneous estimation of Acyclovir, Abacavir, Sofosbuvir and Lopinavir.
Author: Mrs. Rani Divekar
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4403219644