Year: 2024
Type: article
Abstract: Objective and design Chronic kidney disease (CKD) is associated with persistent systemic inflammation. Reduced red blood cell (RBC) survival in patients with CKD has been identified for several decades. The purpose of this study is to explore whether excessive erythrophagocytosis exists and contributes to systemic inflammation in CKD. Methods and subjects A CKD rat model was induced by 5/6 nephrectomy. Erythrophagocytosis was evaluated in vivo and in vitro. RT-qPCR and immunohistochemistry were used to determine the inflammatory effects after erythrophagocytosis. Results Erythrocyte osmotic fragility and deformability progressively declined, and the percentage of PS-exposing RBCs progressively increased in CKD rats. More of erythrocytes from 5/6Nx rats were phagocytosed by macrophages in comparison to those from control rats in vivo and in vitro. Macrophages phagocytosed CKD erythrocytes exhibited higher mRNA levels of IL-6, CXCL-10, CXCL-11, iNOS, IL-1β, ICAM-1 and MCP-1. The red pulp of rats with CKD exhibited higher levels of p-NFκB, IL-6, iNOS and CXCL-10. ELISA results showed significantly increased plasma levels of both IL-6 and CXCL-10 in patients with long-term hemodialysis compared with those in healthy controls. Conclusions Our results indicated that excessive erythrophagocytosis might be an important source of systemic inflammation in CKD.
Source: Journal of Inflammation Research
DOI: 10.2147/jir.s467136
Authors: Qian Meng, Xiaowei Yang, Zhongcheng Liu, Guoxing You, Wanyi Chen, Bing Zhao, Huizi Zhu, Liang Xu, Yan Zhou, Xiang Liu, Chunjuan Zhai, Rong Wang, Lian Zhao, Jing Sun
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,836
Awards: G1733025152, G589787612
OpenAlex ID: W4403224074