Figure S8 from Antiangiogenic Tyrosine Kinase Inhibitors have Differential Efficacy in Clear Cell Renal Cell Carcinoma in Bone
Year: 2024
Type: other
Abstract: Analysis of CD8+ cell infiltration at baseline in bone tumors and description of COMET analysis. A) Representative images captured by confocal microscope of CD8+ cell infiltration in bone tumors and quantification of CD8+ cell number; tumor (green, GFP), CD8+ cells (yellow), blood vessels (laminin, red), nuclei (blue, DAPI); dashed line, tumor edge; mean + SEM, n=3/group; dotted lines. B) Spatial definition of tumor edge; dashed line, tumor edge; dotted lines were draw at + 25 µm of distance from the tumor edge, that represents the tumor interface. C) A quantification of the % of CD8+ cells is shown, mean + SEM, n=3/group. D) Visual representation of experimental design (referred to Figure 4.). E, F) Representative images captured by confocal microscope of the distance (dotted line) between CD8+ cells (yellow) and the closest blood vessel (red, laminin) with quantification, mean + SEM, n=25-75/group. (F). G) Representation of step-by-step process of analysis of the immune infiltrate including tissue segmentation in VisioPharm. P values by one-way ANOVA with Tukey’s HSD post hoc test; p < 0.05 = *. Bar, 25 µm.Veh- vehicle; Axi- axitinib; Cabo- cabozantinib; Lenv- lenvatinib. Lam-laminin; Inner reg-inner region.
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4403226686