Work

Nucleic acid detection with single-base specificity integrating isothermal amplification and light-up aptamer probes

Year: 2024

Type: article

Abstract: We report a novel platform for label-free nucleic acid detection using isothermal amplification and light-up aptamer probes. This assay converts double-stranded amplicons into single-stranded targets to enable sequence-specific hybridization with split dapoxyl aptamer probes, offering attomolar sensitivity and single-base specificity.

Source: Nanoscale

DOI: 10.1039/d4nr01638f

Authors: Jaekyun Baek, Jihyun Park, Youngeun Kim

Institution: Seoul National University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze