Work

Formation of bovine serum albumin–galangin nanoparticles and their potential to inhibit reactive oxygen species–induced inflammation: Ethanol desolvation versus pH-shifting method

Year: 2024

Type: article

Abstract: The pH-shifting method, as an ecofriendly approach, is a promising alternative to the desolvation method, yet systematic comparison of their properties is still lacking. In this study, BSA-galangin nanoparticles (BSA-GA NP) were designed for alleviating reactive oxygen species (ROS)-mediated macrophage inflammation by the 2 separate methods. Compared with the desolvation method, BSA exhibited a higher loading capacity for GA under the pH-shifting method, which was attributed to the exposure of the binding site leading to enhanced affinity for GA and a more compact particle structure. Further analyses evidenced that the electron arrangement and crystal structure of GA changed with different methods. The content of the random coil of BSA was elevated after the pH-shifting method. Additionally, the smaller size rendered the pH-shifting treated BSA-GA NP easier to be taken up by macrophages, and the enhanced specific surface area conferred excellent ROS scavenging and anti-inflammatory performances. This study may provide new insights into the choice of loading methods.

Source: Journal of Dairy Science

DOI: 10.3168/jds.2024-25495

Authors: Weigang Zhong, Lei Xu, Qi Wang, Xue Shen

Institution: Jilin University

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,500