Work

Structural Catalytic Core in Subtilisin-like Proteins and Its Comparison to Trypsin-like Serine Proteases and Alpha/Beta-Hydrolases

Year: 2024

Type: article

Abstract: Subtilisin-like proteins are serine proteases that use two types of catalytic triads: Ser-His-Asp and Ser-Glu-Asp. Here, we investigate the two known families of subtilisin-like proteins, the subtilases (Ser-His-Asp triad) and the serine-carboxyl proteinases (Ser-Glu-Asp triad), and describe the local structural arrangements (cores) that govern the catalytic residues in these proteins. We show the separation of the cores into conserved structural zones, which can be repeatedly found in different structures, and compare the structural cores in subtilisin-like proteins with those in trypsin-like serine proteases and alpha/beta-hydrolases.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms252211858

Authors: Alexander I. Denesyuk, Konstantin Denessiouk, Mark S. Johnson, Vladimir N. Uversky

Institutions: Åbo Akademi University, University of South Florida, USF Health Byrd Alzheimer's Institute

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $3,296

Funders: China Scholarship Council, Turun Yliopisto, Biocenter Finland