Year: 2024
Type: article
Abstract: Reconstructing premortem DNA methylation levels in ancient DNA has led to breakthrough studies such as the prediction of anatomical features of the Denisovan. These studies rely on computationally inferring methylation levels from damage signals in naturally deaminated cytosines, which requires expensive high-coverage genomes. Here, we test two methods for direct methylation measurement developed for modern DNA based on either bisulfite or enzymatic methylation treatments. Bisulfite treatment shows the least reduction in DNA yields as well as the least biases during methylation conversion, demonstrating that this method can be successfully applied to ancient DNA.
Source: Genome biology
Authors: Susanna Sawyer, Pere Gelabert, Benjamin Yakir, Alejandro Llanos-Lizcano, Alessandra Sperduti, Luca Bondioli, Olivia Cheronet, Christine Neugebauer-Maresch, Maria Teschler-Nicola, Mario Novak, Ildikó Pap, Ildikó Szikossy, Tamás Hajdu, Vyacheslav Moiseyev, Andrey Gromov, Gunita Zariņa, Eran Meshorer, Liran Carmel, Ron Pinhasi
Institutions: University of Vienna, Hebrew University of Jerusalem, University of Atlántico, University of Naples - L'Orientale, University of Padua, Austrian Academy of Sciences, Austrian Archeological Institute, Natural History Museum Vienna, University of Primorska, Institute for Anthropological Research, Eötvös Loránd University, University of Szeged, Hungarian Natural History Museum, Peter the Great's Museum of Anthropology and Ethnography, University of Latvia
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,290
OpenAlex ID: W4403282610