Work

Laser Aggregometry Assessment of Blood Microrheology in a Slit Fluidic Channel Covered With Endothelial Cells

Year: 2024

Type: article

Abstract: The blood rheology in vitro in glass or plastic microfluidic chips is different from that in vivo in blood vessels with similar geometry. Absence of vascular endothelium is suggested to cause these discrepancies. This work aims to perform in vitro measurements of blood microrheologic parameters in a slit microfluidic channel covered with endothelial cells (HUVEC). The laser aggregometry was employed to measure the intensity of laser light, backscattered from the blood flow, as a function of shear stress to evaluate the hydrodynamic strength of red blood cells (RBC) aggregates in terms of critical shear stress (CSS). The results demonstrated a decrease in CSS accompanied by an increase in the accuracy of its measurement at similar shear stresses when endothelial cells were present in the channel. The findings hold valuable implications for advanced approaches for endothelization of microfluidic devices, facilitating the study of blood flow dynamics in physiologically more relevant environment.

Source: Journal of Biophotonics

DOI: 10.1002/jbio.202400379

Authors: Alexey N. Semenov, Andrei E. Lugovtsov, Petr B. Ermolinskiy, Alexander V. Priezzhev

Institutions: Lomonosov Moscow State University, Saarland University

Language: English

Topic: Blood properties and coagulation

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,440