Work

Classical Conditioning of Adult Drosophila

Year: 2024

Type: preprint

Abstract: Over the intervening years, advances in genetics have largely changed the focus of studies from the role of single genes in memory to investigation of memory-relevant neuronal circuits. However, the original behavioral paradigms have remained largely unaltered, besides investigators making a few useful tweaks to the training and testing apparatus and modifications to the operating procedures. In this protocol, we provide the reader with a detailed description of the manufacture and assembly of a typical T-maze apparatus, where populations of adult flies can be trained and their odor memory tested later, by giving them a binary choice between the two trained odors. We describe how variations of the training apparatus permit both aversive (odor-shock) and appetitive (odor-sugar) memories to be studied. In addition, we describe a recent modification of the apparatus and protocol that permits study of multisensory (color and odor) aversive and appetitive learning. Control assays for sensory acuity and locomotion are also included.

Source: Cold Spring Harbor Protocols

DOI: 10.1101/pdb.prot108566

Authors: Zeynep Okray, Pedro F. Jacob, John-Paul Moszynski, Clifford B. Talbot, Scott Waddell

Institution: University of Oxford

Language: English

Topic: Neurobiology and Insect Physiology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

Funder: Wellcome Trust

Awards: The connectome of olfactory memory circuits in the adult fly, Operating Principles of Parallel Memory Systems, A high-quality connectome of the complete adult Drosophila central nervous system, Decoding the molecular identity of neurons

OpenAlex ID: W4403298198

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