Impact of Cyclic Loading on the Resistance of Epoxy-treated Bovine Pericardium Modified With Polyvinyl Alcohol to Calcification and Proteolytic Degradation
Year: 2024
Type: article
Abstract: Highlights: Epoxy-treated xenopericardium modified with polyvinyl alcohol has increased resistance to calcification and proteolytic degradation in vitro. Cyclic loads negatively affect fatigue life of studied material, moreover, this process is accompanied by deterioration of its anti-calcium and anti-enzyme properties. Additional studies aimed at improving the wear resistance of the hydrogel in the biological material are required. Aim. To study the resistance of epoxy-treated bovine pericardium modified with polyvinyl alcohol to calcification and proteolytic degradation in collagenase after exposure to cyclic loading. Material and methods. The epoxy-treated patches made with xenopericardium were treated with polyvinyl alcohol according to the original method, after that they were subjected to cyclic loading (70 million cycles) using the HiCycle wear tester system. Visualization of the surface and internal structure of the biomaterial was performed by scanning electron microscopy. The resistance to calcification was assessed by incubating the samples in a solution saturated with calcium ions and phosphate ions for 3 and 6 weeks, followed by quantitative measurement of the calcium by spectrophotometry. The susceptibility of the samples to proteolytic degradation was determined by weight loss after incubation in a solution of clostridial collagenase for 24 hours. The control group consisted of patches of unmodified epoxy-treated bovine pericardium. Results. After cyclic loading, patches of xenopericardium modified with polyvinyl alcohol showed signs of fatigue (cracks on the surface and large cavities in the internal structure). Although cyclic loading significantly worsened the resistance of the modified tissue to calcification and proteolytic degradation (by 3 and 5 times, respectively, relative to the values obtained for patches stored under static conditions), patches from this group showed better results compared to unmodified patches. Thus, they contained 1.5 to 2 times less calcium after 3 and 6 weeks of incubation in a calcium-saturated solution. Mass loss after incubation in collagenase was 1.5 times lower for patches of modified biomaterial undergoing cyclic loading compared to unmodified patches. Conclusion. The proposed modification method of the epoxy-treated xenopericardium with polyvinyl alcohol increases the resistance of biological tissue to calcification and proteolytic degradation. Although cyclic loading negatively affects the protective properties of the polymer coating over time, this type of modification can potentially slow down the degeneration of biomaterial used in manufacturing of bioprosthetic heart valves.
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4403315919