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Protocol for isolating antigen-specific monoclonal antibodies from immunized mice utilizing the Beacon platform

Year: 2024
Type: article
Abstract: Isolation of antigen-specific plasma B cells had been challenging until the recent arrival of the Beacon platform. Leveraging light-sorting technology, Beacon can perform high-throughput screening of plasma B cells on a chip to sort single cells with the desired antigen specificity. Here, we present a protocol for isolating antigen-specific plasma B cells from immunized mice using Beacon, sequencing the encoded B cell receptors (BCRs), and cloning and expressing the resulting antibodies. This protocol can easily be extended to human samples.
DOI: 10.1016/j.xpro.2024.103389
Authors: Meilan Fu, Xiangwei Wang, Shubing Tang, Shimeng Bai, Longfei Ding, Yangyang Hu, Kangli Cao, Tianhan Yang, Chen Zhao, Xiaoyan Zhang, Jianqing Xu
Institutions: Fudan University, Shanghai Public Health Clinical Center, Southern University of Science and Technology, Shenzhen Third People’s Hospital, Shanghai Medical College of Fudan University, Zhongshan Hospital
Language: English
Field: Medicine
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4403324386
FWCI: 0.686
Cites: 10
Cited by: 3
Related to: 10