Single-shot 20-fold expansion microscopy
Year: 2024
Type: article
Abstract: Expansion microscopy (ExM) is in increasingly widespread use throughout biology because its isotropic physical magnification enables nanoimaging on conventional microscopes. To date, ExM methods either expand specimens to a limited range (~4-10× linearly) or achieve larger expansion factors through iterating the expansion process a second time (~15-20× linearly). Here, we present an ExM protocol that achieves ~20× expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative expansion with the simplicity of a single-shot protocol. This protocol, which we call 20ExM, supports postexpansion staining for brain tissue, which can facilitate biomolecular labeling. 20ExM may find utility in many areas of biological investigation requiring high-resolution imaging.
Source: Nature Methods
Authors: Shiwei Mitchell-Wang, Tay Won Shin, Harley B. Yoder, Ryan B. McMillan, Hanquan Su, Yixi Liu, Chi Zhang, Kylie S. Leung, Peng Yin, Laura L. Kiessling, Edward S. Boyden
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $12,290
Funders: National Science Foundation, Howard Hughes Medical Institute, U.S. Department of Health and Human Services, Open Philanthropy Project, Good Ventures Foundation, European Commission, National Institutes of Health, National Institute on Aging, National Institute of Mental Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Biomedical Imaging and Bioengineering, Office of Extramural Research, National Institutes of Health
Awards: G1067232454, G1910030611, G2485429652, G2555497485, Synthetic Ligands for Directing Immune Responses, Mechanisms of pathology and neuronal hyperactivity in a memory circuit in Alzheimer's disease, G3455539091, Graduate Research Fellowship Program, Development of a scalable strategy for reconstructing cell-type determined connectome of the mammalian brain, G524113085, G5724598711, G5747523997, Graduate Research Fellowship Program (GRFP), G6340053574, G6405711847, Expansion Microscopy, Lipid imaging expansion microscopy to study Alzheimer's disease, Multiplexed Nanoscale Protein Mapping Through Expansion Microscopy and Immuno-SABER, G8418944472, G8475582690
OpenAlex ID: W4403338428