Work

Two novel antimicrobial peptides P33-57 and mP168-187 from zebrafish showing potent antibacterial activities

Year: 2024

Type: article

Abstract: It is known that overuse or abuse of antibiotics has undoubtedly accelerated the global antibiotic resistance crisis, and long-time use of antibiotics may have adverse effects on the health of animal, human, and ecosystem. Therefore, it is necessary to find antibiotic alternatives that can be used in aquaculture and are non-toxic to the human. Here we clearly demonstrated that both the PH and FYVE domain of Plekhf2 in zebrafish have antibacterial properties and can interact with PGN in this study. Therefore, we screened four candidate peptides from the two domains. It was demonstrated that P 152-172 and P 168-187 had no obvious antibacterial activities , while P 33-57 and mP 168-187 had strong antibacterial activities, which may be used as antimicrobial peptides . Additionally, transmission electron microscopy experiment revealed that P 33-57 and mP 168-187 can destroy the cell wall of bacteria, thereby directly killing bacteria . Importantly, it was found that P 33-57 and mP 168-187 had no hemolysis to red blood cells and lacked cytotoxicity. In summary, P 33-57 and mP 168-187 could be seen as antibacterial activity centers of PLEKHF2 and may be promising antimicrobial peptides to combat bacterial infections facing an antibiotic-resistance crisis.

Source: Fish & Shellfish Immunology

DOI: 10.1016/j.fsi.2024.109950

Authors: Dongqiu Guo, Hao Wang, Jing He, Liqiao Zhang, Longxiao Liu, Xia Wang

Institutions: Qingdao University of Science and Technology, Chinese Academy of Sciences, Institute of Oceanology

Language: English

Topic: Antimicrobial Peptides and Activities

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,420