Work

Inconsistencies in the published rabbit ribosomal rRNAs: a proposal for uniformity in sequence and site numbering

Year: 2025

Type: article

Abstract: (rabbit) ribosome cryo-EM structures reveals numerous confusing inconsistencies. First, there are a plethora of single-nucleotide differences among the various rabbit 28S and 18S rRNA structures. Second, two nucleotides are absent from the NCBI Reference Sequence for the 18S rRNA gene. Moving forward, we propose using the Broad Institute's rabbit whole-genome shotgun sequence and numbering to reduce modeling ambiguity and improve consistency between ribosome models.

Source: RNA

DOI: 10.1261/rna.080294.124

Authors: Swastik De, Michelle Zhou, Zuben P Brown, Raymond N. Burton-Smith, Yaser Hashem, Tatyana Pestova, Christopher U T Hellen, Joachim Frank

Institutions: Columbia University, Université de Bordeaux, Inserm, Institut Européen de Chimie et Biologie, Acides nucléiques: Régulations naturelles et artificielles, SUNY Downstate Health Sciences University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Alternative mechanisms of different stages in eukaryotic translation, Structural Studies of Macromolecular Assemblies Using Cryo-EM, Mechanisms of eukaryotic translation and ribosome-associated mRNA surveillance and protein quality control

OpenAlex ID: W4408169294

FWCI: 0.298

Cites: 70

Cited by: 1

Related to: 10