Aerosol immunization with influenza matrix, nucleoprotein, or both prevents lung disease in pig
Year: 2024
Type: article
Abstract: Current influenza vaccines are strain-specific and require frequent updates to combat new strains, making a broadly protective influenza vaccine (BPIV) highly desirable. A promising strategy is to induce T-cell responses against internal proteins conserved across influenza strains. In this study, pH1N1 pre-exposed pigs were immunized by aerosol using viral vectored vaccines (ChAdOx2 and MVA) expressing matrix (M1) and nucleoprotein (NP). Following H3N2 challenge, all immunizations (M1, NP or NPM1) reduced lung pathology, but M1 alone offered the greatest protection. NP or NPM1 immunization induced both T-cell and antibody responses. M1 immunization generated no detectable antibodies but elicited M1-specific T-cell responses, suggesting T cell-mediated protection. Additionally, a single aerosol immunization with the ChAdOx vaccine encoding M1, NP and neuraminidase reduced lung pathology. These findings provide insights into BPIV development using a relevant large natural host, the pig.
Source: npj Vaccines
Authors: Eleni Vatzia, Basudev Paudyal, Barbara Dema, Brigid Veronica Carr, Ehsan Sedaghat-Rostami, Simon Gubbins, Bhawna Sharma, Elliot Moorhouse, Susan Morris, Marta Ulaszewska, Ronan MacLoughlin, Francisco J. Salguero, Sarah C. Gilbert, Elma Tchilian
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,690
Funders: Bill and Melinda Gates Foundation, UK Research and Innovation, Department for Environment, Food and Rural Affairs, UK Government, University of Oxford, Directorate for Biological Sciences, Animal and Plant Health Inspection Service, Medical Research Council, Biotechnology and Biological Sciences Research Council
Awards: BBSRC National Bioscience Research Infrastructure: Low Containment Services and Science Platforms, High Containment, G2120815429, G2222632322, Broad and effective protection against influenza achieved by viral vectored vaccines, G3034558885, Host Responses to Viral Infection: Enhancing protective response in vertebrate hosts, G4389917213, G5081335473, G5455636131, Recognition and control of virus infections, G5736889118, G6312107230, G6851372104, University of Oxford - to explore the potential of conserved, internal influenza proteins as vaccine antigens offering broad protection against currently circulating stra..., G7570934350, G8674210727
OpenAlex ID: W4403366775