Taurine effects on cell proliferation and gene expression of apoptosis and oxidative stress in broiler satellite cells under heat stress
Year: 2024
Type: article
Abstract: This study aimed to investigate the effects of taurine on broiler embryonic muscle-derived satellite cells under heat stress. Satellite cells were extracted from the leg muscle of 18-day-old broiler embryos and cultured under two different temperature (37°C and 42°C), with or without taurine addition. The experiment comprised four treatment groups: 37°C without taurine, 37°C with taurine, 42°C without taurine, and 42°C with taurine. Cell survival rates and the expression of genes related to heat shock proteins (HSPs), cell cycle, oxidative stress, and MAPK/ERK-Nrf2 were analyzed, considering the effects of taurine and culture temperature and their interaction. The results showed higher cell survival rates at 42°C, with increased expression of satellite cell markers pax7 and MyoD in all treatment groups. HSP90 and HSP40 gene expressions were significantly upregulated at 42°C, and taurine treatment also led to a significant increase. However, HSP70 expression showed the opposite pattern. In cell cycle-related genes, expect for bcl2, significant differences were observed due to taurine and culture temperature, with an interaction effect observed for p53. Regarding antioxidant-related gene expression, superoxide dismutase was significantly upregulated at 42°C and also in the taurine treatment. extracellular signal-regulated kinase and nuclear factor erythroid 2-related factor 2 showed significant increases and decreases due to 42°C and taurine, respectively, while c-Jun N-terminal kinase and p38 displayed opposing results. These diverse findings suggest that broiler satellite cells can undergo proliferation and growth at 42°C, and taurine treatment can enhance their resilience to heat stress, potentially maintaining cellular health even at 37°C.
Authors: Jinryong Park, Kwanseob Shim, Darae Kang
Institutions: Korea Food Research Institute, Jeonbuk National University
Language: English
Topic: Aldose Reductase and Taurine
Subfield: Cell Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $383
OpenAlex ID: W4403371839