Figure 2 from Peroxisome Proliferator–Activated Receptor δ Suppresses the Cytotoxicity of CD8+ T Cells by Inhibiting RelA DNA-Binding Activity
Year: 2024
Type: other
Abstract: PPARδ negatively regulates the expression of perforin, granzyme B, and IFNγ. A, Western blot expression of PPARδ in naive, activated, and cytotoxic murine or human CD8+ T cells. B, Western blot expression of indicated proteins in Ppard+/+ and Ppard−/− murine CTLs from two groups (two mice for each phenotype in a group) of mice and ELISA expression of IFNγ in the culture media of the CTLs. Data (mean ± SD) represent three independent experiments with similar results. **, P < 0.02. C, Western blot expression of indicated proteins in Ppard+/+ and Ppard−/− murine CTLs transfected with a plasmid expressing GFP or human PPARδ and ELISA expression of IFNγ in the culture media of the CTLs. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. D, Western blot expression of indicated proteins in Ppard+/+ and Ppard−/− murine CTLs treated with 1 μmol/L GW501516 (GW) and ELISA expression of IFNγ in the culture media of GW501516-treated CTLs. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05; **, P < 0.02. E, Western blot expression of indicated proteins in human CTLs transfected with either two different PPARδ siRNAs or a plasmid expressing human PPARδ and ELISA expression of IFNγ in the culture media of the CTLs. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. F, The mRNA levels of indicated genes in the Ppard+/+ and Ppard−/− murine CTLs were measured by real-time PCR. mRNA levels of the Ppard+/+ sample are assigned a value of 1. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. G, The mRNA levels of indicated genes in human CTLs treated with two different PPARδ siRNAs were measured by real-time PCR. mRNA levels of CTLs treated with the s iC are assigned a value of 1. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05; **, P < 0.02. H, The mRNA levels of indicated genes in human CTLs transfected with a plasmid expressing GFP or human PPARδ were measured by real-time PCR. Data (mean ± SD) represent three independent experiments with similar results. Unpaired Student t test was used for statistics. *, P < 0.05; **, P < 0.02.
Authors: Bo Cen, Jie Wei, Dingzhi Wang, Raymond N. DuBois
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4403377525