Figure 5 from Peroxisome Proliferator–Activated Receptor δ Suppresses the Cytotoxicity of CD8+ T Cells by Inhibiting RelA DNA-Binding Activity
Year: 2024
Type: other
Abstract: RelA, IFNγ, and granzyme B are critical for CTL cytolytic activity. A, Western blot expression of indicated proteins in human CTLs transfected with a plasmid expressing RelA or PPARδ and ELISA expression of IFNγ in the culture media of the CTLs. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. B, The cytotoxicity of human CTLs (18-hour incubation, left) and Western blot expression of indicated proteins in HCT116 cells (2-hour incubation, right) after the CTLs transfected with plasmid expressing PPARδ or human RelA were incubated with HCT116 cells. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. C, Western blot expression of indicated proteins in Ppard+/+ and Ppard−/− murine CTLs transfected with a control siRNA (si C) or siRNA targeting RelA (si RelA) and ELISA expression of IFNγ in the culture media of the CTLs. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. D, The cytotoxicity of Ppard+/+ and Ppard−/− murine CTLs (18-hour incubation, left) and Western blot expression of indicated proteins in HCT116 cells (2-hour incubation, right) after Ppard+/+ and Ppard−/− CTLs transfected with a control siRNA (si C) or siRNA targeting RelA (si RelA) were incubated with HCT116 cells. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. E, The cytotoxicity of human CTLs (18-hour incubation, left) and Western blot expression of indicated proteins in HCT116 cells (2-hour incubation, right) after the CTLs treated with a control antibody (Ig G) or antibody against IFNγ (α-IFNγ), or granzyme B (α-GZMB), or the combination of the two antibodies were incubated with HCT116 cells. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05; **, P < 0.02. F, A hypothetical model of PPARδ interactions with RelA in the nucleus of CTLs. C-PARP, cleaved PARP; C-caspase 3, cleaved caspase 3; C-caspase 7, cleaved caspase 7.
Authors: Bo Cen, Jie Wei, Dingzhi Wang, Raymond N. DuBois
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4403377553