Figure 1 from Peroxisome Proliferator–Activated Receptor δ Suppresses the Cytotoxicity of CD8+ T Cells by Inhibiting RelA DNA-Binding Activity
Year: 2024
Type: other
Abstract: PPARδ inhibits CD8+ T-cell activation in vivo and cytolytic activity in vitro. A, Proliferation of CFSE-labeled CD8+ T cells from small intestine tumors and adjacent normal tissues in control and GW501516-treated mice. B, Percentage of IFNγ-expressing CD8+ T cells in small intestine tumor-specific and adjacent normal in control and GW501516-treated mice by flow cytometry. C, Number of small intestine tumors in different size groups in control and GW501516-treated mice. For A–C, the results were obtained from two independent experiments using three mice for each group in each experiment. D, The cytotoxicity of Ppard+/+ and Ppard−/− murine CTLs (18-hour incubation, left) and Western blot expression of indicated proteins in mouse colorectal cancer cell lines CT26 and MC38 cells (2-hour incubation, right) after CTLs were incubated with colorectal cancer cells. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. E, The cytotoxicity of Ppard+/+ and Ppard−/− murine CTLs (18-hour incubation, left) and Western blot expression of indicated proteins in CT26 cells (2-hour incubation, right) after CTLs transfected with plasmid expressing GFP or human PPARδ were incubated with CT26 cells. Data (mean ± SD) represent three independent experiments with similar results. *, P < 0.05. C-PARP, cleaved PARP; C-caspase 3, cleaved caspase 3; C-caspase 7, cleaved caspase 7.
Authors: Bo Cen, Jie Wei, Dingzhi Wang, Raymond N. DuBois
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4403377753