Chronopotentiometric Stripping Analysis of Proteins and Their Interactions
Year: 2024
Type: article
Abstract: Chronopotentiometric constant current stripping analysis (CPS) is a highly sensitive method for protein analysis that does not require modification or labeling. During the CPS analysis, proteins yield the so-called peak H, resulting from the catalytic hydrogen evolution reaction. This peak is sensitive to both local and global changes in protein structure, allowing the study of individual proteins, as well as their complexes. CPS analysis has been utilized in studying biomedically important proteins involved in neurodegenerative diseases and cancer. In this article, we describe the development of CPS protein analysis and its progress over the past decade. We also demonstrate the versatility of the method and its potential applications.
Source: Chemické listy
DOI: 10.54779/chl20240515
Authors: Tatiana Staroňová, Veronika Ostatná
Institution: Czech Academy of Sciences, Institute of Biophysics
Language: English
Subfield: Electrochemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4403388249