Impacts of Hyperglycemia on Epigenetic Modifications in Human Gingival Fibroblasts and Gingiva in Diabetic Rats
Year: 2024
Type: article
Abstract: Periodontal disease is considered one of the diabetic complications with high morbidity and severity. Recent studies demonstrated the involvement of the epigenome on diabetic complications. Histone modifications change chromatin architecture and gene activation. Histone modifications have been reported to alter chromatin structure and regulate gene transcription. In this study, we investigated the impacts of H3 lysine 4 trimethylation (H3K4me3) and specific histone methyltransferases of H3K4 methylation, su(var)3-9, enhancer-of-zeste, and trithorax domain 1A (SETD1A) on periodontal tissue affected by the diabetic condition. We observed the increase in H3K4me3 and SETD1A in gingival tissue of diabetic rats compared with the normal rats. Cultured human fibroblasts (hGFs) confirmed a high glucose-induced increase in H3K4me3 and SETD1A. We further demonstrated that high glucose increased the gene expression of matrix metalloproteinase (MMP) 1 and MMP13, which were canceled by sinefungin, an SETD1A inhibitor. Our investigation suggests that diabetes triggers histone modifications in the gingival tissue, resulting in gingival inflammation. Histone modifications may play crucial roles in the development of periodontal disease in diabetes.
Authors: Kento Kojima, Nobuhisa Nakamura, Airi Hayashi, Shun Kondo, Megumi Miyabe, Takeshi Kikuchi, Noritaka Sawada, Tomokazu Saiki, Tomomi Minato, Reina Ozaki, Sachiko Sasajima, Akio Mitani, Keiko Naruse
Institution: Aichi Gakuin University
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,296
Awards: Mechanisms of the progression of periodontitis in diabetes via the activation of polyol pathway, 糖尿病に歯周病が合併することによる糖尿病性神経障害への影響とそのメカニズムの解明, G6745655631, Effects of three subtypes of Mfa1 fimbriae on epithelial-mesenchymal transition in periodontal tissue destruction., Development of predictors of coronary artery disease development using expression of the adipocytokine, chemerin.
OpenAlex ID: W4403403415