Molecular Characterization of polyhydroxybutyrate producers from different mangrove forest soil rhizosphere of Kerala
Year: 2024
Type: article
Abstract: The mangrove ecosystem is deemed a rare environment exemplified by fluctuating tidal flows, scarcity of nutrients, hypoxia, high salinity, and ultraviolet radiation.Stressful conditions form reactive oxygen species (ROS) such as superoxide radicals, hydroxyl radicals, hydrogen ABSTRACT The ecological impact of non-bio-based plastics has accelerated the demand for natural bio-based resins like polyhydroxyalkanoates (PHAs), biodegradable compounds with industrial and medical applications.This study focuses on the isolation and molecular identification of PHB-synthesizing bacteria from mangrove forest ecosystems in Kerala, India.Thirty-two microbial species were isolated from four mangrove rhizospheric soil sites.Sudan Black B and Nile Blue A staining confirmed lipid inclusions and PHB accumulation in sixteen isolates.Bacillus velezensis, identified through Crotonic acid quantification and 16S rRNA sequence analysis, showed the highest PHB concentration.Phylogenetic analysis revealed Bacillus species dominated the mangrove soil biome.This is the first report of PHB-synthesizing bacteria isolated from Kerala's mangrove forests for potential biopolymer production.
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
SDG: Life on land
Open Access status: gold
OpenAlex ID: W4403422603