Figure 2 from Oncogenic Mutant p53 Sensitizes Non–Small Cell Lung Cancer Cells to Proteasome Inhibition via Oxidative Stress–Dependent Induction of Mitochondrial Apoptosis
Year: 2024
Type: other
Abstract: Onc-p53 NSCLC cells exhibit basal and PI-induced oxidative stress. A, Average oxidized (GSSG) vs. reduced (GSH) levels of GSH obtained from metabolomic profiles (DepMap) of NSCLC cell lines that either express Onc-p53 protein (Onc-p53) or lack p53 protein expression due to homozygous deletion/frameshift/premature termination mutations (loss of function; LOF). Data were analyzed using the unpaired t test. B, GSH concentration was determined in lysates of the indicated cell lines. C, Relative GSH concentrations were determined in lysates of H1975-Control vs. H1975-p53KO cells. D, Levels of ROS were determined in the indicated cell lines using a fluorescent ROS detection assay and plotted as mean fluorescence units (MFU). E, H460 or H1975 cells were treated with vehicle, BTZ (5 nmol/L), NAC (1 mmol/L), or BTZ + NAC for 24 hours. Relative ROS levels normalized to vehicle treatment for each cell line were determined by staining with DCFH-DA followed by flow cytometry. F, H1975 cells were treated as per E for 48 hours, and cell viability was assessed by trypan blue exclusion. All pairwise comparisons were made using the Student t test. *, P < 0.05; **, P < 0.01; ***, P < 0.005. Error bars indicate ± 1.0 SD.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4403433604