Protocol to measure cell avidity between cord blood-derived NK cells and leukemia cell line KG-1a
Year: 2024
Type: article
Abstract: The avidity between immune cells and target cells is crucial for effective immune responses. Here, we present a protocol to measure single-cell avidity between cord blood-derived natural killer (NK) cells and the human acute myelocytic leukemia cell line KG-1a. We describe the steps for isolating cord blood mononuclear cells, purifying NK cells, and culturing KG-1a cells. We then detail the procedures for detecting cell avidity between NK cells and KG-1a cells using the acoustic force-based z-Movi cell avidity analyzer. For complete details on the use and execution of this protocol, please refer to Wang et al. 1 • Steps for isolating mononuclear cells from cord blood • Magnetic purification of primary NK cells from cord blood • Measuring the avidity between NK cells and suspended target cells using z-Movi Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The avidity between immune cells and target cells is crucial for effective immune responses. Here, we present a protocol to measure single-cell avidity between cord blood-derived natural killer (NK) cells and the human acute myelocytic leukemia cell line KG-1a. We describe the steps for isolating cord blood mononuclear cells, purifying NK cells, and culturing KG-1a cells. We then detail the procedures for detecting cell avidity between NK cells and KG-1a cells using the acoustic force-based z-Movi cell avidity analyzer.
Source: STAR Protocols
Institution: University of Science and Technology of China
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Awards: G3348329717, G6859026141
OpenAlex ID: W4403455022