Work

Identification of novel antifreeze peptides from yak skin gelatin ultrasound-assisted enzymatic hydrolysate

Year: 2024

Type: article

Abstract: In this paper, a new type of antifreeze peptide was identified from the ultrasonic-assisted enzymolysis product of yak skin. First, the antifreeze peptides were obtained by ultrasonic-assisted enzymolysis of yak skin gelatin. The obtained peptides with 150 W ultrasound treatment could increase the survival rate of Lactobacillus plantarum from 16 % to 60 % after four freeze-thaw cycles. Then, the component with the highest antifreeze activity in the peptides' ultrafiltration product was analyzed by LC-MS/MS to obtain 961 peptides. After screening of antifreeze activity and physical properties, three antifreeze peptide sequences were obtained (S1: GERGGPGGPGPQ, S2: PGGAEGPGRDAQQP, S3: VAPPGAPKKEH). DSC analysis, Lactobacillus plantarum cryoprotection experiments and molecular docking showed that the S1 sequence had the best antifreeze activity. This study provides a new idea for the high-value utilization of yak by-products and a potential candidate for food antifreeze agents.

Source: Ultrasonics Sonochemistry

DOI: 10.1016/j.ultsonch.2024.107102

Authors: Xiaotong Ma, Wenxing Wang, Hongmei Shi, Xiangying Kong, Li Zhang

Institutions: Gansu Agricultural University, Gansu Provincial Agriculture and Animal Husbandry

Language: English

Topic: Protein Hydrolysis and Bioactive Peptides

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,700

Funder: Natural Science Foundation of Gansu Province