Figure 5 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Year: 2024
Type: other
Abstract: Tracing of TRP demonstrates that AT-0174, a dual TDO2/IDO1 inhibitor, decreases TRP catabolism. A, Schematic of the study design: cells in attached vs. forced suspension culture were incubated in 13C11-labeled TRP for 24 hours, and then suspended cells were treated with vehicle control (DMSO) or 1 µmol/L (“+”) or 10 µmol/L AT-0174 (“++”) for 24 or 48 hours at which time cells were harvested, lysed, and analyzed by MS. Intracellular heavy (labeled) TRP catabolites (dark blue) and light (unlabeled) TRP catabolites including (B) TRP, (C) formyl-KYN, (D) KYN, (E) 3-hydroxykynurenine, (G) kynurenic acid, and (F) NAD were measured and are represented as peak area. Biological replicates (n = 3) were conducted for each group, and data are displayed as mean ± SEM with two-way ANOVA analysis. *, P < 0.05; **, P < 0.01; **, P < 0.001; ****, P < 0.0001.
Language: English
Subfield: Biological Psychiatry
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4403467405