Figure 1 from Blocking Tryptophan Catabolism Reduces Triple-Negative Breast Cancer Invasive Capacity
Year: 2024
Type: other
Abstract: Inflammatory cytokines induce heterogeneous TDO2 expression in TNBC cells. A, IHC of TDO2 protein in MDA-MB-453 cells treated with vehicle or 10 ng/mL IL1β + TNFα for 48 hours. B, TDO2 protein in MDA-MB-453 treated with IL1β + TNFα or vehicle control for 48 hours and detected by immunoblotting. C, t-SNE graph showing TDO2 in scRNA-seq on MDA-MB-453 cells treated with vehicle or IL1β + TNFα for 48 hours. D, Volcano plot showing gene fold change following vehicle or IL1β + TNFα treatment (P < 0.05; FDR < 0.05) with twofold or greater up- (red) or downregulated (blue) gene sets. E, Significant upregulated/downregulated genes identified using GSEA. F, scRNA-seq expression of TRP catabolism–associated genes TDO2, KMO, and KYNU in MDA-MB-453 treated with vehicle or IL1β + TNFα. G,TDO2 measured by qRT-PCR in three TNBC cell lines in response to vehicle or IL1β + TNFα treatment. Data are displayed as mean ± SD by two-way ANOVA analysis. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. KMO, kynurenine 3-monooxygenase; NES, normalized enrichment score; t-SNE, t-distributed stochastic neighbor embedding; UMAP, Uniform Manifold Approximation and Projection.
Language: English
Subfield: Biological Psychiatry
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4403467732