Check my sample sheet: a web application for validating illumina sample sheets before demultiplexing
Year: 2026
Type: article
Abstract: Proper configuration of an Illumina sequencing run is crucial for ensuring successful outcomes, with the sample sheet serving as a vital component by summarizing sample metadata. Accurate demultiplexing and downstream analysis depend heavily on the correctness of this sample sheet. To address this need, validation of the sample sheet becomes essential to prevent errors during sequencing processes. We present a browser-based web application built using Streamlit, which incorporates the IEM Python module from the Sequana Python library to perform comprehensive sanity checks on Illumina sample sheets. This application allows users to upload or paste sample sheet files through a simple interface that requires no installation, where automated validation checks are performed. These checks assess key parameters such as sample duplication, consistency, and adherence to Illumina formatting standards. HierHGT-DTI provides an effective multiscale relation-aware framework for cold-start DTI predictiConclusions. The “Check My Sample Sheet" web application enables users to preemptively detect and resolve potential issues in their sample sheets, reducing errors and improving sequencing outcomes. The application supports both the legacy bcl2fastq (v1) and the newer bclconvert (v2, BCL Convert) sample sheet formats, detecting the version automatically. Additionally, the application’s open-source nature supports extensibility, making it adaptable to specific workflow requirements.
Source: BMC Bioinformatics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,990
Funder: Agence Nationale de la Recherche
OpenAlex ID: W4403475296