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Protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion solution

Year: 2024
Type: article
Abstract: Human primary cardiomyocytes are a valuable in vitro model for studying human heart physiology and disease. Here, we present a protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion solution. We describe steps for sample collection, tissue pre-processing, and cell dissociation. We then detail procedures for calcium reintroduction and viability assessment. This protocol can significantly improve the efficiency of cardiomyocyte isolation and subsequent cell function, making them an even more reliable model for cardiac research. For complete details on the use and execution of this protocol, please refer to Shi et al. 1 • Instructions for digesting and collecting adult human primary cardiomyocytes • Steps for gentle reintroduction of calcium into cardiomyocytes • Guidance on assessing cardiomyocyte viability following isolation Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Human primary cardiomyocytes are a valuable in vitro model for studying human heart physiology and disease. Here, we present a protocol for the dissociation of adult human primary cardiomyocytes using a methylcellulose-supplemented digestion solution. We describe steps for sample collection, tissue pre-processing, and cell dissociation. We then detail procedures for calcium reintroduction and viability assessment. This protocol can significantly improve the efficiency of cardiomyocyte isolation and subsequent cell function, making them an even more reliable model for cardiac research.
DOI: 10.1016/j.xpro.2024.103379
Authors: Xun Shi, Yafei Huang, Bingying Zhou
Language: English
Field: Medicine
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4403477174
FWCI: 0.267
Cites: 2
Cited by: 1
Related to: 10