Year: 2024
Type: article
Abstract: Metabolic dysfunction in the liver represents a predominant feature in the early stages of alcohol-associated liver disease (ALD). However, the mechanisms underlying this are only partially understood. To investigate the metabolic characteristics of the liver in ALD, we did a relative quantification of polar metabolites and lipids in the liver of mice with experimental ALD using untargeted metabolomics and untargeted lipidomics. A total of 99 polar metabolites had significant abundance alterations in the livers of alcohol-fed mice. Pathway analysis revealed that amino acid metabolism was the most affected by alcohol in the mouse liver. Metabolites involved in glycolysis and the TCA cycle were decreased, while glycerol 3-phosphate (G3P) and long-chain fatty acids were increased. Relative quantification of lipids unveiled an upregulation of multiple lipid classes, suggesting that alcohol consumption drives metabolism toward lipid synthesis. Results from enzyme expression and activity detection indicated that the decreased activity of mitochondrial glycerol 3-phosphate dehydrogenase contributed to the disordered metabolism.
Source: Journal of Proteome Research
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1486559928, G1578363164, Dietary Fat and Alcoholic Liver Disease, G3068197408, G4273066803, G564347165, G5849146513, G7119938101, Ultra Performance Liquid Chromatography High Resolution High Mass Accuracy Mass Spectrometer, G7783371710, G8030251470, G8942210600, G9000150761, The Role of Nutrition in the Development/Progression of Alcohol-Induced Organ Injury, RNA Modification Changes in Alcohol-Associated Liver Disease, Hepatobiology and Toxicology COBRE, Integrated therapies for alcohol use and ALD (ITAALD) Network -UofL Clinical Center, Intestine FXR activation by LGG-derived nanoparticles in alcohol-associated liver disease
OpenAlex ID: W4403481081