Year: 2024
Type: article
Abstract: Our research elucidates the cleavage processes of the RNase III enzyme, DICER, which plays a crucial role in the production of small RNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs). Utilizing high-throughput dicing assays, we expose the bipartite pairing rule that dictates the cleavage sites of DICER. Furthermore, we decode the intricate recognition mechanism of the primary YCR motif and identify an analogous secondary YCR motif that influences DICER's cleavage choices. Collectively, our findings clarify the bipartite pairing rule and enhance our understanding of the role of RNA motifs in modulating DICER's cleavage activity, laying the groundwork for future research on their roles in miRNA biogenesis and gene regulation. DICER is a vital enzyme in small RNA production. Here the authors show how bipartite pairing rules and dual YCR motifs guide DICER's activity on precursor microRNAs.
Source: Nature Communications
Authors: Thi Nhu-Y Le, Cong Truc Le, Tuan Anh Nguyen
Institution: Hong Kong University of Science and Technology
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,790
OpenAlex ID: W4403482270