Year: 2024
Type: article
Abstract: In order to illustrate the epigenetic heterogeneity, versatile tools of single-cell ChIP-seq (scChIP-seq) are necessary to meet the convenience and accuracy. Here, we develop MobiChIP, a compatible ChIP-seq library construction method based current sequencing platform with single cell level. As a novel capture strategy, MobiChIP is efficient to capture the fragments from tagmented nuclei of numerous species and execute the mixing of samples from different tissues or species. Especially, this strategy enables the flexible sequencing manipulation and sufficient nucleosome amplification without customized sequencing primers. MobiChIP reveals the landscape of chromatin regulation regions with active(H3K27ac) and repressive(H3K27me3) histone modification markers in peripheral blood mononuclear cells (PBMCs), and accurately unveiled the epigenetic repression of hox gene cluster in PBMCs than ATAC-seq. Meanwhile, we complete the bioinformatics pipeline to integrates the scChIP-seq data and scRNA-seq to illustrate the cellular epigenetic and genetic heterogeneity. One-Sentence Summary A high-throughput single-cell ChIP-seq based droplet reveals the integration of scRNA-seq data and scChIP-seq data.
Source: Molecular Omics
DOI: 10.1039/d4mo00111g
Authors: Xianhong Yu, Guantao Zheng, Liting Xu, Weiyi Guo, Guodong Chen, Yiling Zhu, Tingting Li, Mingming Rao, Linyan Wang, Rong Cong, Hao Pei
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4403482385