Bacteriophage Dosing and Its Effect on Bacterial Growth Suppression in a Staphylococcus epidermidis Model: An In Vitro Study
Year: 2024
Type: article
Abstract: Background: Phages are an emerging therapy in the treatment of prosthetic joint infections, though many challenges remain, including an incomplete understanding of optimal phage dosing. Materials and Methods: We performed an in vitro assessment of how phage dosing as measured by multiplicity of infection (MOI) impacts bacterial growth in planktonic and biofilm conditions using a Staphylococcus epidermidis model. Staphylococcus epidermidis ATCC 35984 was combined in planktonic and biofilm forms with phage vB_SepM_Alex at varying concentrations, and growth was monitored via spectrophotometry. Results: Planktonic bacterial growth was significantly higher when MOI ≤ 0.01 compared with MOI ≥ 10 ( p < 0.05). Biofilms with phage dosing at ≤ 10 4 plaque-forming units (PFU)/mL had significantly greater spectrophotometer readings than those dosed at 10 10 PFU/mL ( p < 0.05). Conclusions: Our findings suggest lower, not higher, phage dosing is associated with greater bacterial persistence. Our study helps inform the dosing and delivery of this alternative form of antibiosis.
Source: PHAGE
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4403494770