MetaMax: an objective-sized instrument for advanced light microscope characterization and empirical metadata collection.
Year: 2024
Type: preprint
Abstract: MetaMax is a device designed to replace the objective lens in light microscopes and provide a wide range of experimental metadata that characterize microscope performance. MetaMax includes a large-area photodiode that is referenced against calibrated power meters to measure excitation light power and source stability; a broadband LED to quantify detector system responsivity and signal-dependent noise; a spectrometer to identify excitation wavelengths; an adjustable iris to simulate an objective’s back aperture; and a quadrant photodiode to assess beam alignment and aperture overfill. MetaMax enables users to collect performance data for both quality control and comprehensive acquisition metadata. It simplifies access to performance data that typically requires expert knowledge and expensive equipment to obtain, lowering the access barrier for users. Such performance data is crucial for improving the reliability and reproducibility of microscopy images. The MetaMax aims to facilitate collaboration through data reusability, and promote a more rigorous, transparent approach to scientific inquiry using light microscopy techniques. This work presents a detailed evaluation and comparison of several MetaMax prototypes.
Authors: Selene Flemming, David Grunwald
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
OpenAlex ID: W4403501184