Work

Evaluation of riboflavin, nanocurcumin, and hydrogen peroxide under light conditions: Reduction of mature dental biofilms and enamel mineral loss

Year: 2024

Type: article

Abstract: • Natural photosensitizers mediated PDT has a positive antibacterial effect against Streptococcus mutans and lactobacillus acidophilus dual-species biofilms. • PDT based on natural photosensitizers and hydrogen peroxide showed no significant effect on enamel mineral loss. • PDT could be a complementary method to combat dental caries. Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of Streptococcus mutans and Lactobacillus acidophilus dual-species biofilms and their effect on the enamel mineral loss. The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser ( n = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). While HP-PDT showed a reduction of 37% ( p < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, p < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups. The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria.

Source: Photodiagnosis and Photodynamic Therapy

DOI: 10.1016/j.pdpdt.2024.104379

Authors: Shima Afrasiabi, Ahmed Qays Khudhur AL． Gburi, Ladan Ranjbar Omrani, Nasim Chiniforush, Zohreh Moradi

Institution: Tehran University of Medical Sciences

Language: English

Topic: Photodynamic Therapy Research Studies

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,450