Work

Protocol for oil red O staining of low-density lipoproteins for in vivo cell treatment

Year: 2024

Type: article

Abstract: Low-density lipoproteins (LDLs) are the most abundant circulating lipoproteins and the most critical factor in the development of atherosclerosis. This protocol allows the staining of LDLs with oil red O to monitor particle uptake in bright-field microscopy. Here, we describe how to stain isolated LDLs using oil red O and how to use them to monitor LDL uptake in time-lapse experiments or fixed cells. • Protocol to stain human LDLs using oil red O • Guide for a time-lapse experiment and to monitor LDL uptake in living cells • Steps for analyzing LDL uptake in formaldehyde-fixed cells in a slide glass Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Low-density lipoproteins (LDLs) are the most abundant circulating lipoproteins and the most critical factor in the development of atherosclerosis. This protocol allows the staining of LDLs with oil red O to monitor particle uptake in bright-field microscopy. Here, we describe how to stain isolated LDLs using oil red O and how to use them to monitor LDL uptake in time-lapse experiments or fixed cells.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2024.103376