A broadly reactive ultralong bovine antibody that can determine the integrity of foot-and-mouth disease virus capsids
Year: 2024
Type: article
Abstract: Foot-and-mouth disease vaccination using inactivated virus is suboptimal, as the icosahedral viral capsids often disassemble into antigenically distinct pentameric units during long-term storage, or exposure to elevated temperature or lowered pH, and thus raise a response that is no longer protective. Furthermore, as foot-and-mouth disease virus (FMDV)'s seven serotypes are antigenically diverse, cross-protection from a single serotype vaccine is limited, and most existing mouse and bovine antibodies and camelid single-domain heavy chain-only antibodies are serotype-specific. For quality control purposes, there is a real need for pan-serotype antibodies that clearly distinguish between pentamer (12S) and protective intact FMDV capsid. To date, few cross-serotype bovine-derived antibodies have been reported in the literature. We identify a bovine antibody with an ultralong CDR-H3, Ab117, whose structural analysis reveals that it binds to a deep, hydrophobic pocket on the interior surface of the capsid via the CDR-H3. Main-chain and hydrophobic interactions provide broad serotype specificity. ELISA analysis confirms that Ab117 is a novel pan-serotype and conformational epitope-specific 12S reagent, suitable for assessing capsid integrity.
Source: Journal of General Virology
DOI: 10.1099/jgv.0.002032
Language: English
Subfield: Agronomy and Crop Science
Domain: Life Sciences
Open Access status: hybrid
Funders: Bill and Melinda Gates Foundation, Wellcome Trust, UK Research and Innovation, National Institute for Health and Care Research, Directorate for Biological Sciences, Medical Research Council, Engineering and Physical Sciences Research Council, Biotechnology and Biological Sciences Research Council, Biomedical Research Council, NIHR Oxford Biomedical Research Centre
Awards: G1080657738, Human Genetics and Disease Biology: Core Renewal for the Wellcome Trust Centre for Human Genetics, Intestinal homeostasis: identification of the factors that influence the balance between regulatory and effector cells in the intestine and how alterations in these can lead to chronic intestinal inflammation., G1294961325, G1491676723, G1596336884, G1713228754, G1732642323, High Containment, G2219541475, G2222632322, G2281224446, G2567570596, G285427424, G2873296756, G2919144128, Science Services, G3387191942, G3513052630, G3816277877, G3873106536, G3983353637, Disease Pathogenesis, G5175273828, G5455636131, Structural Studies on Viruses, Viral Proteins and Cell Interactions, G5621985085, Recognition and control of virus infections, G6054759104, G6312107230, G646469678, The Pirbright Institute - to identify and apply FMDV-specific antibodies to improve vaccine quality, G6738704210, The Oxford Interdisciplinary Bioscience Doctoral Training Partnership, Human Genetics and Disease Biology: Core Renewal for the Wellcome Trust Centre for Human Genetics, G73532689, G7459592443, G8290657359, G8674210727, Low Containment, G8934771875, G981451289
OpenAlex ID: W4403539427