A Critical View on the Use of DNA Hydrogels in Cell‐Free Protein Synthesis
Year: 2024
Type: article
Abstract: Numerous studies have reported in the past that the use of protein‐encoding DNA hydrogels as templates for cell‐free protein synthesis (CFPS) leads to better yields than the use of conventional templates such as plasmids or PCR fragments. Systematic investigation of different types of bulk materials from pure DNA hydrogels and DNA hydrogel composites using a commercially available CFPS kit showed no evidence of improved expression efficiency. However, protein‐coding DNA hydrogels were advantageously used in microfluidic reactors as immobilized templates for repetitive protein production, suggesting that DNA‐based materials offer potential for future developments in high‐throughput profiling or rapid in situ characterization of proteins.
Source: Angewandte Chemie
Authors: Svenja Moench, Phillip Lemke, Abbey Hansen, Christoph Bickmann, Martin Peng, Kersten S. Rabe, Carmen M. Domínguez, Christof M. Niemeyer
Institution: Karlsruhe Institute of Technology
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,250
Funder: Helmholtz Association
OpenAlex ID: W4403551798