Work

Upcycling olive pomace into pectic elicitors for plant immunity and disease protection

Year: 2024

Type: article

Abstract: Olive oil production generates substantial quantities of pomace, which are often disposed of in soil, leading to adverse effects on agriculture and the environment. Furthermore, climate change exacerbates plant diseases and promotes the use of toxic phytochemicals in agriculture. However, olive mill wastes can have high potential as reusable and valuable bioresources. Using diluted ethanol, an environmentally friendly solvent, we extracted a fraction containing short and long oligogalacturonides, short arabino-oligosaccharides and polysaccharides. The obtained extract elicited key features of plant innate immunity in Arabidopsis seedlings, including the phosphorylation of mitogen-activated protein kinases MPK3 and MPK6 and the upregulation of defence genes such as CYP81F2 , WRKY33 , WRKY53 , and FRK1 . Notably, pretreatment of adult Arabidopsis and tomato plants with the olive pomace extract primed defence responses and enhanced their resistance to the phytopathogens Botrytis cinerea and Pseudomonas syringae . Our results highlight the opportunity to upcycle the two-phase olive pomace collected at the late stage of olive oil campaign, in low-cost and sustainable glycan elicitors, contributing to reducing the use of chemically synthesized pesticides. • Oligogalacturonides and arabino-oligosaccharides were extracted from olive pomace. • The extract triggers MAPK phosphorylation and induces defense genes in Arabidopsis. • Pretreatment with the extract primes Arabidopsis immunity to Botrytis and Pseudomonas. • Application of the extract on tomato enhances resistance to Pseudomonas. • Pomace collected in late milling stages is ideal for valorisation.

Source: Plant Physiology and Biochemistry

DOI: 10.1016/j.plaphy.2024.109213