Protocol for developing shape-morphing 4D bioprinted magnetic constructs to promote articular cartilage regeneration using silk fibroin-gelatin bioink
Year: 2024
Type: article
Abstract: External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioinks for articular cartilage regeneration. We illustrate the steps for magnetic bioink formulation, bioprinting, and chondrogenic induction of human bone marrow mesenchymal stem/stromal cells. We detail the steps to actuate the constructs using an external magnetic field and then characterize chondrogenesis. Magnetic field actuation may be helpful for mechanically activating constructs for articular cartilage regeneration. For complete details on the use and execution of this protocol, please refer to Chakraborty et al. 1 • Protocol for 4D bioprinting shape-morphing magnetic constructs • Steps for forming bioink using silk fibroin-gelatin and magnetic nanoparticles • Instructions for actuating magnetic constructs by an external magnetic field • Details on molecular characterization of chondrogenesis Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. External magnetic fields can regulate cellular responses. Here, we present a protocol to fabricate magnetic constructs by 4D bioprinting with shape-morphing properties using silk fibroin-gelatin bioink for articular cartilage regeneration. We illustrate the steps for magnetic bioink formulation, bioprinting, and chondrogenic induction of human bone marrow mesenchymal stromal cells. We detail the steps to actuate the constructs using an external magnetic field and then characterize chondrogenesis. Magnetic field actuation may be helpful for mechanically activating constructs for articular cartilage regeneration.
Source: STAR Protocols
Institutions: Indian Institute of Technology Delhi, Maastricht University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,500
OpenAlex ID: W4403626220