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Tetracycline but not sulfamethazine inhibits early root growth of wild grassland species, while seed germination is hardly affected by either antibiotic

Year: 2024

Type: article

Abstract: Seed germination and early growth of grassland species might be influenced by veterinary antibiotics that are extensively released into agricultural habitats. Therefore, we tested impacts of the commonly used antibiotics tetracycline and sulfamethazine, single and in mixture, on seed germination and seedling root growth of six typical species of temperate European grasslands ( Carum carvi , Centaurea jacea , Galium mollugo , Plantago lanceolata , Silene latifolia, Dactylis glomerata ). In standardised germination experiments, we assessed three germination variables (germination percentage, mean germination time, synchrony of germination) and one post-germination variable (seedling root length) under different environmentally realistic antibiotic concentrations (0.1, 1, 10 mg l −1 and a water control). While the germination variables were only irregularly and weakly affected by both antibiotics, seedling root length was strongly reduced by tetracycline, but not by sulfamethazine. Among the test species, D. glomerata was most sensitive to tetracycline with the average root length reduced up to 81 % in the 10 mg l −1 treatment. Its germination behaviour, however, was almost insensitive to the two antibiotics. Mixture effects were only shown in relation to the germination of single species, where the binary mixture produced effects but not the two single antibiotics or, conversely, effects of single antibiotics were lost in the mixture. These findings highlight the potential threat of plant regeneration from seed by veterinary antibiotics, particularly affecting early root growth and potentially influencing plant population growth in natural habitats. • Impact of tetracycline and sulfamethazine on wild grassland species was tested. • Seed germination was only irregularly affected by both veterinary antibiotics. • Seedling root length was strongly reduced by tetracycline but not by sulfamethazine. • There was little evidence for mixture effects of the two antibiotics. • Populations of grassland species could be jeopardized by antibiotic-contaminated livestock manure.

Source: Environmental Pollution

DOI: 10.1016/j.envpol.2024.125178